详情介绍
Nanog Polyclonal Antibody
产品信息
同义词
NANOG
反应物种
Human, Mouse, Rat
宿主物种
Rabbit
免疫原
Recombinant fusion protein of human Nanog(NP_079141.2).
基因ID
79923
同义词
NANOG
蛋白编码
Q9H9S0
抗体分型
IgG
推荐稀释比
WB,1:1000 - 1:2000|IHC,1:50 - 1:200
应用
WB,IHC
Calculated MW
32kDa/34kDa
Observed MW
48kDa
illustration
Western blot analysis of extracts of NIH/3T3 cells, using Nanog antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 45s.|Immunohistochemistry of paraffin-embedded Human testicular seminoma using Nanog Rabbit pAb at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 45s.|Immunohistochemistry of paraffin-embedded Human testicular seminoma using Nanog Rabbit pAb at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
该品牌专注于抗体平台建设12年,引进抗体制备技术,不断优化抗体平台,拥有小鼠单克隆抗体产品线、兔多克隆抗体产品线以及兔单克隆抗体产品线,投放市场的抗体达到10000多种,广泛的应用于IHC、IF、Western Blot、ELISA、ICC/IF、Flow Cyt等科研领域,这些产品已经覆盖了人类基因组靶标;一抗过5000种抗体经过KD/KO验证。
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备注:图片仅供参考。仅供科研,不可用于临床。
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