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详情介绍
Caspase-9 Polyclonal Antibody
产品信息
同义词
CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
反应物种
Human, Mouse, Rat
宿主物种
Rabbit
免疫原
Recombinant fusion protein of human Caspase-9(NP_001220.2).
基因ID
842
同义词
CASP9;APAF-3;APAF3;ICE-LAP6;MCH6;PPP1R56;caspase-9;casp9;Caspase 9
蛋白编码
P55211
抗体分型
IgG
推荐稀释比
WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:100
应用
WB, IHC, IF/ICC
Calculated MW
17kDa/30kDa/36kDa/46kDa
Observed MW
36kDa/46kDa
illustration
Western blot analysis of extracts of various cell lines, using Caspase-9 antibody at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.|Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.|Immunohistochemistry of paraffin-embedded rat heart using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded human lung cancer using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.|Western blot analysis of extracts of Jurkat cells, using Caspase-9 antibody at 1:1000 dilution.Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.|Immunohistochemistry of paraffin-embedded rat heart using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded human lung cancer using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded mouse kidney using Caspase-9 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunofluorescence analysis of NIH/3T3 cells using Caspase-9 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
该品牌专注于抗体平台建设12年，引进抗体制备技术，不断优化抗体平台，拥有小鼠单克隆抗体产品线、兔多克隆抗体产品线以及兔单克隆抗体产品线，投放市场的抗体达到10000多种，广泛的应用于IHC、IF、Western Blot、ELISA、ICC/IF、Flow Cyt等科研领域，这些产品已经覆盖了人类基因组靶标；一抗过5000种抗体经过KD/KO验证。
天津俊达生物科技有限公司，是从事实验室耗材销售的现代化生物科技企业，公司本着“诚信为本，互利共赢"的核心价值观，秉承“以市场为导向，提供服务"的宗旨为各大院校、科研单位、第三方实验室检测机构等提供的产品和服务。目前公司主要经营分子生物学、细胞生物学、免疫学等生物学产品，多种实验室耗材 欢迎垂询！
备注：图片仅供参考。仅供科研，不可用于临床。
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