详情介绍
KO Validated Bax Polyclonal Antibody
产品信息
同义词
BCL2L4;BAX
反应物种
Human, Mouse, Rat
宿主物种
Rabbit
免疫原
Recombinant fusion protein of human Bax(NP_620116.1).
基因ID
581
蛋白编码
Q07812
抗体分型
IgG
推荐稀释比
WB,1:500 - 1:1000|IF/ICC,1:50 - 1:100|IP,1:50 - 1:200
应用
WB, IF/ICC, IP
Calculated MW
4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW
23kDa
illustration
Western blot analysis of extracts of various cell lines, using Bax antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.|Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.|Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Bax antibody . Western blot was performed from the immunoprecipitate using Bax antibody at a dilution of 1:1000.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.|Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.|Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Bax antibody . Western blot was performed from the immunoprecipitate using Bax antibody at a dilution of 1:1000.
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备注:图片仅供参考。仅供科研,不可用于临床。
[KO Validated] Bax Polyclonal Antibody抗体
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