mTOR Polyclonal Antibody抗体

反应物种Human, Mouse, Rat

宿主物种Rabbit

免疫原Recombinant fusion protein of human mTOR(NP_004949.1).

基因ID2475

蛋白编码P42345

抗体分型IgG

推荐稀释比WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

应用

WB, IHC, IF/ICC

Calculated MW

288kDa

Observed MW

288kDa

illustration

Western blot analysis of K562, using MTOR antibody at aQ1731 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.|Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody at dilution of 1:150 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded human placenta using mTOR antibody at dilution of 1:150 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunohistochemistry of paraffin-embedded mouse kidney using mTOR antibody at dilution of 1:150 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.|Immunofluorescence analysis of C6 cells using mTOR Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.|Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.